mAb anti-Human IL-8, I8-60, Detector

Description: Mouse monoclonal antibody to human interleukin 8 (IL-8)

Purification: Protein G affinity purified

Product Type: Primary antibody, detection antibody in matched antibody pair

Target Protein: Human IL-8

Immunogen: Purified recombinant human IL-8

Fusion Myeloma: P3X63Ag8.653

Specificity: This antibody recognizes both recombinant and native human IL-8.

Species Reactivity: Human, others not tested.

Cross-reactivity: This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized in 0.01M PBS, pH 7.0.

Reconstitution: Double distilled water is recommended and to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20oC

Research Area: Cytokine, interleukin and chemotaxis

Background:

Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens.  IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens.  IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis.  IL-8 is also known to promote angiogenesis.

Applications:

1. ELISA: This antibody can be used as detection antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MO-C40017A).  

2. Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5mg/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating that the antibody’s neutralization activity is specific.  The antibody also neutralized IL-8 induced calcium influx in human granulocytes.

3. Western Blot: The antibody used at 0.02-0.1μg/mL concentrations will allow visualization of 100ng/lane of recombinant human IL-8.  

Figure: Western blot analysis of recombinant human IL-8 using clone I8-60.

4. IHC:  The antibody was used to detect IL-8 expression in the stratum corneum of psoriatic skin tissue.  The image below shows the result of detecting with anti-IL-8 clone 60, biotinylated 2rd antibody and avidin-HRP.  The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.