mAb anti-Human IL-8, I8-60, Tracer (Biotin Labeled)

Description: Biotin conjugated mouse monoclonal antibody to human interleukin 8 (IL-8)

Purification: Protein G affinity purified

Product Type: Tracer antibody in matched antibody pair, biotin conjugated.

Target Protein: Human IL-8

Immunogen: Purified recombinant human IL-8

Fusion Myeloma: P3X63Ag8.653

Specificity: This antibody recognizes both recombinant and native human IL-8.

Species Reactivity: Human, others not tested.

Cross-reactivity: This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).

Host / Isotype: Mouse, IgG1 Kappa

Storage Buffer Formulation: 0.01M PBS, pH 7.2  in 1% gelatin and 0.1% proclin-300.

Storage: Store at -20oC. Avoid repeated freeze and thaw cycles.

Research Area: Cytokine, interleukin and chemotaxis

Background:

Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens.  IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens.  IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis.  IL-8 is also known to promote angiogenesis.

Applications:

ELISA: In combination with a monoclonal capture antibody (Cat No: MO-C40017A) and avidin-HRP conjugate, this biotin conjugated antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.