mAb anti-HP CagA, 10E9,

Description: Mouse monoclonal antibody (mAb) to H. pylori cytotoxin associated gene A (CagA) protein

Purification: Protein G affinity purified

Product Type: Primary antibody, capture antibody in matched antibody pair

Target Protein: H. pylori cytotoxin associated gene A protein

Immunogen: A 32KD recombinant protein cloned from the highly immunogenic region of H. pylori cytotoxin associated gene A.

Fusion Myeloma: Sp2/0-Ag14

Specificity: The mAb reacts with H. pylori CagA

Cross-Reactivity: Cross-reaction with other proteins has not been found.

Host / Isotype: Mouse, IgG2a Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area: Bacteriology

Background:  

Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach.  In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer.  The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors.  CagA is used as a biomarker for virulent H. pylori strains.

Application: 

ELISA:  Clone 10E9 coated plate selectively reacts with cell lysate of a CagA containing H. pylori strain whenHRP conjugated anti-CagA clone 3C10 is used as detection antibody.  In addition, clone 10E9 coated wells react with recombinant CagA antigen in combination withHRP conjugated anti-CagA mAb clone 5C6, clone 3C10, or clone 3C1.

Western Blot:  This clone detects the 32 KD recombinant CagA antigen (immunogen).

References: 

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