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Description
Details
Description
Endo-β-N-acetylglucosaminidase H (Endo H) is a recombinant glycosidase that can cleave the chitobiose core structure of high-mannose and certain hybrid-type oligosaccharides in N-glycoproteins, removing N-linked high-mannose from glycoproteins. This product is derived from Streptomyces plicatus and is recombinantly expressed in E. coli. It has no additional endo- and exo- glycosidase activities and can be used for the complete deglycosylation of antibodies or other glycoproteins.
Unit definition: One unit (U) is defined as the amount of the enzyme required to remove more than 95% of the carbohydrates from 10 μg of denatured RNase B at 37 °C for 60 minutes.
Storage solution: 20mM Tris-HCl，50mM NaCl，5mM EDTA，pH 7.5
Product components
|
Components |
Size1 |
Size2 |
|
Recombinant Endo H |
3KU |
15KU |
|
10× Endo H reaction buffer |
500µl |
500µl |
|
10× glycoprotein denaturation buffer |
500µl |
500µl |
Additional
Additional Information
| Product Specificity | Recombinant Endo H |
|---|---|
| Application | Complete removal of N-linked hypermannose of antibodies or other glycoproteins. |
| Size | No |
