mAb anti-Human Phospho-CREB [p-S133], B1C4

Description: Mouse monoclonal antibody against serine133 phosphorylated human cAMP response element-binding protein (p-S133-CREB)

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Serine133 phosphorylated human cAMP response element-binding protein (p-S133-CREB)

Immunogen: Serine133 phosphorylated human CREB peptide fragment conjugated with KLH

Fusion Myeloma: Sp2/0-Ag14

Specificity: Reactive to serine133 phosphorylated human CREB peptide fragment, and reactive to KLH or BSA conjugated serine133 phosphorylated human CREB peptide fragment.  Not reactive to un-conjugated KLH or BSA.

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG2 Kappa

Formulation: Lyophilized form from a solution of 0.01M PBS, pH7.0

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC.  Avoid freeze and thaw cycles.

Research Area: Signal transduction, gene transcription

Background:

cAMP response element-binding protein (CREB) is a transcription factor that regulates the transcription of a wide spectrum of genes in response  to second massagers (cAMP and Ca++) and cell growth or stress signals.  The primary binding sites ofCREBon the promoter regions are cAMP response element (CRE) and calcium response element which have a common 5’-TGACGTCA-3’ motif.  CREBcan be phosphorylated and activated by cAMP-dependent protein kinase A, calcium/calmodulin-dependent kinase, and kinases in the mitogen-activated kinase (MAPK) and extracelluar signal-regulated kinase (ERK) signal cascades.

CREB plays an important role in neuroplasticity and memory formation by controlling the expression of c-fos, brain-derived neurotrophic factor, tyrosine hydrolase and many neuropeptides. 

Application: 

In indirect ELISA, the antibody is reactive to serine133 phosphorylated humanCREBpeptide and reactive to serine133 phosphorylated humanCREBpeptide conjugated with KLH or BSA.  The peptide and the conjugates were coated at 1μg/ml in pH 9.5 carbonate buffer.

Other applications have not been evaluated.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.