mAb anti Human PD-L1, clone 6A5

Description: Mouse monoclonal antibody (mAb) to human programmed death- ligand 1 (PD-L1), or mouse mAb to B7H1, or mouse mAb to human CD274.

Purification: Protein G affinity purified

Target Protein: Human programmed death-ligand 1 (PD-L1), also known as B7H1 or CD274.

Immunogen: Human cell expressed recombinant PD-L1 (Phe19-Arg238) with poly-histidine tag at C-terminus.

Fusion Myeloma: SP2/0

Specificity: Reactive with human programmed death- ligand 1 (PD-L1).

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to reconstitute the antibody to 1mg/mL.

Storage: Store at -20^oC

Research Area: Immunosuppression. Cancer immunotherapy.

Background:

Programmed Death-Ligand 1 (PD-L1), a 40KD transmembrane protein, is a ligand of immune checkpoint protein Programmed Death-1 (PD-1). During T-cell receptor signalling, the binding of PD-L1 to PD-1 transmits an inhibitory signal to activated T-cells to decrease their proliferation. PD-L1 is also believed to play a role in the generation and regulation of Treg cells, and in the enhancement of Treg cell function. PD-L1/PD-1 pathway is important to immune suppression, such as self-tolerance, suppression of immune-rejection during pregnancy and allograft, and immune evasion by cancers. Upregulation of PD-L1 has been found to be associated with many cancer types. For example, increased PD-L1 expression has been shown to be related to tumor aggressiveness and higher mortality in patients with renal cell carcinoma, and related to significantly poorer prognosis and lower intraepithelial CD8+ T-lymphocyte count in patience with ovarian cancer. Antibodies that block the PD-L1 /PD-1 pathway have been extensively studied in clinical trials, and some have been approved as immunotherapy for cancer.  

Applications: 

ELISA: Indirect ELISA: The antibody reacts with recombinant PD-L1 in indirect ELISA. 

Western blot: Western Blot: Anti-PD-L1 clone 6A5 detected 40ng recombinant PDL-1 on Western Blot. The blot was stained with HRP substrate 4-Chloro-1-Naphthol.

References:

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