mAb anti-Human FPRL-1 2nd extracellular loop, 3D7

Description: Mouse monoclonal antibody against the 2nd extracellular loop of human formyl peptide-receptor-like-1 (FPRL1)

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: The 2nd extracellular loop of human formyl peptide-receptor-like-1 (FPRL1)

Immunogen: A short peptide (ASWGGTPEERLKC) corresponding to the amino acids on the 2nd extracellular loop of human FPRL1 is conjugated with KLH for immunization

Fusion Myeloma: Sp2/0-Ag14

Specificity: This antibody is reactive to the peptide (ASWGGTPEERLKC) from the 2nd extracellular loop of human FPRL1 and is also reactive to intact human FPRL1. 

Species Reacitvity: Human

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC. Avoid repeated freeze and thaw cycles.

Research Area: G-protein coupled membrane receptor, chemotaxis and Alzheimer’s disease

Background:

Human formyl peptide receptor-like 1(FPRL1) is a G-protein coupled seven transmembrane receptor.  The receptor can bind to the formyl peptides derived from the degradation of the cell wall of infected bacteria and host cells.  FPRL1 expressed on the cell surface of neutrophils and other leukocytes mediates the chemotaxis of these cells toward infected site.  It has been reported that acute phase protein SAA is also a ligand of FPRL1 and SAA can induce the secretion of IL-8 and TNF-a by neutrophil through FPRL1 signalling.  FPRL1 has also been shown to mediate the chemotaxis of microglia by Abeta42 peptide and the internalization of Abeta42 peptide into cytoplasma of macrophage, indicating that FRPL1 may participate in the Abeta42 peptide elicited pathogenesis in Alzheimer’s disease. 

Application: 

ELISA: In indirect ELISA, the antibody is reactive to the peptide (ASWGGTPEERLKC) from the 2nd extracellular loop of human FPRL1.   

Western Blot: Western blot analysis of extracts from 293 cells transfected with FPRL1 DNA using anti-FPRL1 antibody (3D7) at 1:1000 (1μg/mL) dilution.

 

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.