pAb Rabbit anti-Human PML [Ring Finger Domain]

Description: Rabbit anti-human promyelocytic leukemia protein (PML) ring finger domain polyclonal antibody

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: The ring finger domain in human PML protein. The ring finger, like B box1, is retained in PML/RARa chimera.

Immunogen: Recombinant partial human PML containing the ring finger domain

Specificity: This antibody recognizes normal PML proteins of lower molecular weight, and also recognizes the high molecular weight PML/RARα chimeric protein in promyelocytic leukemia patients.

Species Reacitvity: Human, others not tested

Host: Rabbit

Formulation: Lyophilized in pH 7.4, 0.01M PBS

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20^oC

Research Area: Life science research, Oncology

Background:  

Acute promyelocytic leukemia (PML) is a type of acute myelogenous leukemia that is caused by the translocation of the retinoic acid receptor alpha (RARa) gene at chromosome 17 to the promyelocytic leukemia (PML) gene at chromosome 15. The chimeric protein (PML/RARa) has a higher molecular weight than normal PML protein.  The altered proteins block DNA transcription and differentiation of granulocytes, reduce the production of red cells and platelets, consequently, resulting in anemia and thrombocytopenia.

Application: 

Western Blot (see image): Western blot analysis of NB4 cell lysate using 0.2 μg/mL rabbit anti-PML polyclonal antibody (Cat. N.: AI70002A).  The band shows on the blot is the 106KDa chimeric PML/RARα protein.

Immunofluorescence (see image): Staining of NB4 cells using 2μg/mL anti-PML polyclonal antibody (Cat. No.: AI70002A).

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.