mAb anti-Human PTMA, 4G2

Description: Mouse monoclonal antibody to human prothymosin alpha (PTMA)

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Human prothymosin alpha (PTMA)

Immunogen: A short peptide (eeaengrdapangnan) derived from PTMA was conjugated with KLH for immunization.

Fusion Myeloma: Sp2/0-Ag14

Specificity: This antibody is reactive to PTMA peptide (eeaengrdapangnan) and GST tagged recombinant PTMA protein.

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area: Chromatin-remodelling, cell proliferation, oncology

Background:  

PTMA (prothymosin alpha) is the precursor of thymosin α1.  It is an acidic, non-histone nuclear protein with 109-111 amino acids.  PTMA is implicated with cell cycle regulation, chromatin remodeling, expression of oxidative stress-response genes and immuno-modulation.  PTMA is elevated in malignances such as breast cancer, gastric cancer, prostate and bladder cancers.  PTMA is a potential biomarker for the prognosis of cancer treatment. 

Application: 

Western blot (see image):  This antibody is reactive to GST tagged 38.4KD recombinant PTMA protein.    

The GST tagged recombinant PTMA was loaded at 200ng/lane.  The anti-PTMA clone 4G2 was used at 1μg/ml and the HRP conjugated goat anti-mouse IgG was used at 1:2,000 dilution. 

ELISA: This antibody is reactive to recombinant PTMA protein.

References:

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