mAb anti-Human PSA, CHYH1, Capture

Description: Mouse monoclonal antibody to Human Prostate Specific Antigen (PSA)

Purification: Protein G affinity purified

Product Type: Primary antibody - Capture antibody in matched antibody pair

Target Protein: Human Prostate Specific antigen (PSA)

Immunogen: Human Prostate Specific antigen (PSA)

Fusion Myeloma: Sp2/0-Ag14

Specificity: This antibody is reactive to normal prostate, benign prostate hyperplasia and prostate malignant carcinoma. 

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to reconstitute the antibody.

Storage: Store at -20^oC

Research Area: Oncology

Background:  

Prostate specific antigen (PSA) is a kallikrein related peptidase secreted by the epithelial cells of the prostate gland. Majority of PSA exists in plasma in bounded forms with α1 anti-chymotrypsin or other proteins. The protein is elevated in prostatic disorders such as prostate cancer and benign prostatic hyperplasia. PSA has been used as a biomarker for prostate cancer screening.

Application:

ELISA:  This antibody can be used as a capture antibody in sandwich ELISA applications for humanPSA detection in combination with a tracer antibody (HRP-conjugated, Cat. No.: MO-T40081T) that is derived fromPSA mAb CHYH2 (Cat. No.: MO-T40081B). 

Immunohistochemistry:  The antibody can be used in IHC application for formalin-fixed, paraffin embedded and frozen tissue sections.

Western Blot (see image): Detect PSA antigen (100ng/well) using 1ug/mL mouse anti-human PSA mAb clone CHYH1.

References: 

1. Sadagopan Krishnan et al.  Attomolar Detection of a Cancer Biomarker Protein in Serum by Surface Plasmon Resonance Using Superparamagnetic Particle Labels* Angew. Chem. Int. Ed. Engl. 2011 February 1; 50(5): 1175–1178.

2. Vigneshwaran Mani et al.  Ultrasensitive Immunosensor for Cancer Biomarker Proteins using Gold Nanoparticle Film Electrodes and Multienzyme-Particle Amplification.  ACS Nano. Mar 24, 2009; 3(3): 585–594.

3. Mani V et al.  Highly efficient binding of paramagnetic beads bioconjugated with 100,000 or more antibodies to protein-coated surfaces.  Anal Chem. 2012 Dec 4;84(23):10485-91.

4. Bhaskara V. Chikkaveeraih et al.  Single-Wall Carbon Nanotube Forest Arrays For Immunoelectrochemical Measurement of 4 Protein Biomarkers for Prostate Cancer. Anal. Chem. Nov 1, 2009; 81(21): 9129-9134.

5. Bhaskara V. Chikkaveeraiah et al. Microfluidic Electrochemical Immunoarray for Ultrasensitive Detection of Two Cancer Biomarker Proteins in Serum.  Biosens Bioelectron. Jul 15, 2011; 26(11): 4477-4483.

6. Jeong-Mi Moon et al. A nanowire-based label-free immunosensor: Direct incorporation of a PSA antibody in electropolymerized polypyrrole. Biosensors and Bioelectronics. Volume 57, 15 July 2014, Pages 157–161.

7. Sardesai NP, Barron JC, Rusling JF.Carbon nanotube microwell array for sensitive electrochemiluminescent detection of cancer biomarker proteins. Anal Chem 2011; 17(83):6698-703.

8. Liu D, Yang J, Wang HF, Wang Z, Huang X, Wang Z, Niu G, Hight Walker AR, Chen X. Glucose oxidase-catalyzed growth of gold nanoparticles enables quantitative detection of attomolar cancer biomarkers. Anal Chem 2014; 12(86):5800-6.

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.