mAb anti human IL-18 clone 10H4, Tracer (Biotin Labeled)

Description: Biotin labeled mouse monoclonal antibody to human interleukin-18(IL-18)

Product Type: Tracer, biotin labeled detection antibody in matched antibody pair.

Purification: Protein G affinity purified

Target Protein: Human IL-18

Immunogen: Purified, human cell expressed recombinant human IL-18 

Fusion Myeloma: SP2/0

Specificity: Reactive with human cell expressed IL-18

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG2b Kappa

Formulation: In pH 7.2, 0.01M PBS containing 0.45% gelatin 

Storage: Store at -20^oC.  Avoild freeze and thaw cycles.

Research Area: Cytokine, inflammatory and auto-immune responses

Background:

IL-18, also known as interferon- inducing factor, is a pro-inflammatory cytokine produced by macrophage and other cells. Like interleukin-12, the expression of IL-18 can be stimulated by bacterial protein lipopolysaccharide (LPS) and can induce the production of interferon- by natural killer cells and certain T-cells. It has also been shown that IL-18 can inhibit the IL-4 dependent IgE and IgG1 production and increase IgG2a expression by B-cells. An interleukin-18-binding protein that is expressed by mononuclear cells, can bind IL-18 and inhibit its pro-inflammatory function. It has been reported that IL-18 is implicated in Hashimoto’s thyroiditis, age-related macular degeneration and Alzheimer’s disease.  

Applications: 

Sandwich ELISA: In combination with human IL-18 capture antibody clone 2A10 (Cat. No.: MO-C40094A), this biotin labeled anti-human IL-18 antibody clone 10H4 can be used for quantitative detection of human IL-18 levels in Sandwich ELISA application.  

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.