mAb anti-HCV NS-4, 7B7/5G9

Description: Mouse monoclonal antibody to human hepatitis C virus (HCV) non-structural protein NS-4

Purification: Protein G affinity purified

Product Type: Primary Antibody

Target Protein: Human hepatitis C virus (HCV) non-structural protein NS-4

Immunogen: A synthetic polypeptide of at least 90 residues in length, selected from the immunodominant NS-4 region of Chinese HCV strains

Fusion Myeloma: Sp2/0-Ag14

Specificity: mAb 7B7/5G9 is reactive with synthetic NS-4a (residues 1689-1735 on HCV polyprotein) and recombinant chimeric HCV (60 kDa). 

Species Reactivity: Human hepatitis C virus, others not tested

Cross-Reactivity: No cross reaction can be seen with HCV core region and other non-structural regions.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area: Virology

Background:  

Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission.  HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein).  The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2).  The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein.  HCV viral proteins are produced after processing the polyprotein.  Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.

Application: 

ELISA: mAb 7B7/5G9 is reactive with synthetic NS-4a (residues 1689-1735) in indirect ELISA.

Western Blot: mAb 7B7/5G9, at concentration of 0.5μg/mL will allow visualization of 0.5-1.0μg/lane synthetic NS-4a, and 0.1μg/lane recombinant chimeric HCV. The mAb works on blots transferred from both reducing and non-reducing PAGE gel.

References: 

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