mAb anti-Human B2MG, D2A10

Description: Mouse monoclonal antibody to human beta 2 microglobulin (B2MG)

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Human beta 2 microglobulin

Immunogen: Highly purified beta 2 microglobulin from human urine

Fusion Myeloma: Sp2/0-Ag14

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2.

Reconstitution: Double distillated water is recommended to reconstitute the antibody.

Storage: Store at -20^oC

Research Area: Renal disease, plasma cell cancer

Background:  

Human beta 2 microglobulin (B2MG) is a component of major histocompatibility complex 1 (MHC1).  It is abundant at the surface of lymphocytes and exists at the surface of all cell types except red cells and platelets.  B2MG is normally filtered out of blood through glomeruli, but the process is impaired in patients with renal diseases.  Elevated B2MG level is implicated with lymphoma, multiple myeloma, renal diseases, viral infections and auto-immune conditions.

Application: 

1. ELISA: The antibody reacts with human B2MG.

2. Western Blot: Detect recombinant human B2MG antigen (100ng/lane) using anti-human B2MG mAb D2A10 at 1μg/mL dilution. 

 

3. Immuno-fluorescence staining: 

Stainning of methanol-fixed Hela cells: Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2A10) 1:50 (20ug/mL).

4. Fluorescence Flow Cytometry: 

Above is the histogram of Mouse anti-Human beta 2 Micro Globulin monoclonal antibody (D2A10) 1:100 (10ug/mL) staining of Hela cells.  Black line histogram represents the isotype control, Mouse anti-TB 38Kd antigen monoclonal antibody (MO-I40021D, B12F8).

References:

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