mAb anti-HP CagA, 3C10,

Description: Mouse monoclonal antibody (mAb) to H. pylori cytotoxin associated gene A (CagA) protein

Purification: Protein G affinity purified

Product Type: Primary antibody, detection antibody in matched antibody pair

Target Protein: H. pylori cytotoxin associated gene A protein

Immunogen: A 32KD recombinant protein cloned from the highly immunogenic region of H. pylori cytotoxin associated gene A.

Fusion Myeloma: Sp2/0-Ag14

Specificity: The mAb reacts with H. pylori CagA.

Cross-Reactivity: Cross-reaction with other proteins has not been identified

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area: Bacteriology

Background:  

Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach.  In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer.  The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors.  CagA is used as a biomarker for virulent H. pylori strains.

Application: 

ELISA:  HRP conjugated 3C10 selectively detects cell lysate of a CagA containing H. pylori strain in sandwich ELISA assay when clone 10E9 is used as coating antibody.  In addition, HRP conjugated clone 3C10 can detect the recombinant CagA antigen in sandwich ELISA if use anti-CagA clone 10E9 or clone 5H10 coated ELISA wells.

References: 

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.