mAb anti-His Tag, 10A12

Description: Mouse monoclonal antibody against synthetic 6 x Histidine tag

Product Type: Purified primary antibodies

Target Protein: His-tagged recombinant protein

Immunogen: Synthetic 6 x Histidine peptide conjugated with KLH

Fusion Myeloma: Sp2/0-Ag14

Specificity: This monoclonal antibody binds to His-tagged recombinant protein, but not with KLH.

Cross-Reacitvity: Not identified

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized in pH 7.2 0.01M PBS

Reconstitution: Reconstitute with appropriate volume of dIH₂O to reach a final concentration of 1.0 mg/mL.

Storage: Store at -20 ^oC

Research Area: Life science

Application: 

Western blot: Western blotting using anti-His antibodies (HisH8 and 10A12) to detect His-tagged HIV p24.  

Citations  

1. Liao YT, Kuo SC, Lee YT, Chen CP, Lin SW, Shen LJ, Fung CP, Cho WL, Chen TL. Sheltering effect and indirect pathogenesis of carbapenem-resistant Acinetobacter baumannii in polymicrobial infection. Antimicrob Agents Chemother 2014; 7(58):3983-90.