mAb anti Human IL-17A clone 2D11

Description: Mouse monoclonal antibody to human interleukin-17A (IL-17A)

Purification: Protein G affinity purified

Target Protein: Human IL-17A

Immunogen: Purified, human cell expressed recombinant human IL-17A 

Fusion Myeloma: SP2/0

Specificity: Reactive with recombinant human IL-17A

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to reconstitute the antibody to 1mg/mL.

Storage: Store at -20^oC

Research Area: Cytokine, inflammatory and auto-immune responses

Background:

IL-17A is an inflammatory cytokine produced by Th17 cells, a newly characterised group of CD4 T help cells that plays a role in autoimmune diseases and chronic inflammatory diseases. IL-23 is the main inducer of Th17 cell development. Other cytokines such as TGF-beta, IL-6 and IL-21 were also found to contribute to the procedure. IL-17A belongs to the IL-17 family which includes IL-17A, IL-17B, IL-17C, IL-17D and IL-17E. IL-17, like interferon-gamma, is a potent mediator of delayed-type reaction. IL-17 induces the secretion of many other pro-inflammatory cytokines and chemokines such as IL-6, IL-8, GM-CSF, G-CSF, TGF-beta, TNF-alpha, GRO-alpha, and MCP-1. IL-17 stimulates the expression of NF-kappaB, mitogen activated protein kinase, and prostaglandings by many cell types, and enhances the production of nitric oxide.  

Applications: 

Neutralization:  IL-17A antibody clone 2D11 inhibited IL-17A induced IL-6 expression in human dermal fibroblast.  The IC50 was less than 0.25mg/ml.  

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.