mAb anti Human IL-15 clone 5E8, Capture

Description: Mouse monoclonal antibody to human interleukin-15(IL-15)

Product Type: Primary antibody, capture antibody in matched antibody pair.

Purification: Protein G affinity purified

Target Protein: Human IL-15

Immunogen: Purified recombinant human IL-15 

Fusion Myeloma: SP2/0

Specificity: Reactive with recombinant human IL-15

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to reconstitute the antibody to 1mg/mL.

Storage: Store at -20^oC

Research Area: Cytokine, inflammatory and auto-immune responses

Background:

Interleukin (IL)-15 is an inflammatory cytokine that can induce tumor-necrosis factor-α, IL-1β and inflammatory chemokines. It acts on T cells and natural killer cells through receptors on these cells. IL-15 and IL-2 share common receptor β and γ units (IL-15/IL-2Rβγc) and thus some overlapping functions in stimulating T-cell proliferation and cytokine secretion. However, both cytokines have its own specific receptor α (IL-15Rα or IL-2Rα) that links to distinct signaling pathways.  For example, IL-15 can inhibit self-tolerance mediated by IL-2 mediated activation-induced T cell death, and promote CD8+ memory T-cell survival. Increased IL-15 expression has been reported in patients with psoriasis and other inflammatory autoimmune diseases. IL-15 exists as soluble form, as well as membrane-bound form in which it could be directly bound to the cellular membrane or presented by IL-15Rα receptor. The main mechanism of IL-15 signaling is trans-presentation which is initiated by IL-15 binding to IL-15Rα. The IL-15/IL-15Rα complex subsequently presents IL-15 to surrounding cells bearing IL-15Rβγc complex on their cell surface. Recent research showed that combined activation with IL-12, IL-15, and IL-18 can induce memory-like natural killer (NK) cells that live longer and exhibit enhanced functionality when they encounter a secondary stimulation. The progress has led to increased interest in using IL-15 for cancer therapy.  

Applications: 

Sandwich ELISA: In combination with anti-human IL-15 detection antibody clone B1H6 (Cat. No.: MO-C40095B), clone 5E8 can be used as capture antibody for quantitative measurement of IL-15 in ELISA.  

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.