Recombinant Trypsin Plus (MS grade), Lyophilized

Description

Trypsin is a serine endopeptidase secreted by the pancreas. Its recognition sites are lysine or arginine in proteins, and the cleavage sites are at the carboxyl-terminal of lysine or arginine residues. This product is an improved version of recombinant porcine trypsin expressed in Pichia pastoris, produced in an environment compliant with GMP regulations. It contains no animal-derived components, eliminating the risk of contamination by animal-borne viruses (such as swine influenza virus and porcine parvovirus). Naturally lacking chymotrypsin activity, it is more suitable for rapid digestion and identification of proteins. The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, as well as metal ion chelators such as EDTA.

• Unit definition：One USP unit was defined as a 0.003 increase in absorption at 253 nm per minute of enzymatic digestion of BAEE in 3.2 ml of reaction system (1 cm optical path) at 25 °C, pH 7.6.
• Reconstitution solution：1mM HCl.

Product components