pAb Rabbit anti-Phospho-NF-қB-p65 (Ser468)

Description: Rabbit anti-Serine 468 phosphorylated NF-κB p65 polyclonal antibody

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Serine 468 phosphorylated NF-κB p65

Immunogen: A synthetic peptide (FTDLAS_pVDNSE) derived from Serine 468 phosphorylated NF-κB p65, conjugated with KLH for immunization 

Specificity: This antibody is reactive to the synthetic NF-κB-p65 peptide (FTDLAS_pVDNSE).

Species Reacitvity: Mouse, human and other species with consensus serine 468 phosphorylated NF-κB-p65 peptide (FTDLAS_pVDNSE)

Source: Rabbit

Formulation: Lyophilized in pH 7.4, 0.01M PBS

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area: Life science research, stem cell factor signalling, cancer research

Background:

NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a transcription factor that controls the transcription of many genes that are associated with immuno-response, anti-apoptosis, neuroplasticity and memory. There are five NF-κB proteins in mammals: RelA (NF-κB-p65), RelB, c-Rel, NF-kB (NF-κB-p105), and NF-κB2 (NF-κB-p100). They form a variety of homodimers and heterodimers, each of which activates its own distinctive set of genes.  P65 has been shown to be involved with DNA binding.  The phosphorylation of p65 at Ser468 is inducible by TNF. The phosphorylation mediates p65 ubiquitination and the termination of related gene expression.

Application:

ELISA: The antibody (approx. 1:50,000 -1:120,000 diluted) can detect the serine 468 phosphorylated NF-κB p65 peptide coated on ELISA plate at 100ng/well. 

Other applications have not been evaluated. 

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.