mAb anti-Human PD-1 / CD279, 2H7

Description: Mouse monoclonal antibody (mAb) to human programmed cell death-1 (PD-1), or mouse mAb to human CD279. 

Purification: Protein G affinity purified

Product Type: Primary antibody.

Target Protein: Human programmed cell death-1 (PD-1)

Immunogen: 40kDa recombinant human programmed cell death-1 (PD-1) from ACRO Biosystems (Cat. No.: PD1-H5221).

Fusion Myeloma: Sp2/0-Ag14

Specificity: Reactive with recombinant human programmed cell death-1 (PD-1).  Reactivity with natural PD-1 has not been evaluated.

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area: Immunosuppression

Background: 

Programmed Cell Death-1 (PD-1), also called CD279, is a 288 amino acid cell surface protein that belongs to the immunoglobulin superfamily.  The protein is expressed on T cells, B cells and macrophages.  When PD-1 is joined with its ligand (CD274 or B7-H1), the immune cell’s activation and effector function are attenuated.  Research suggested that PD1 pathway is mainly involved with attenuation at periphery sites while the B7/CTLA-4 pathway is primarily involved with the attenuation of the activation of immune cells in secondary lymphoid organs.  PD-1 pathway is implicated with cancer’s immune-evasion, autoimmunity, and Listeria monocytogenes infection.

Application:

1. Indirect ELISA:  The antibody is reactive to recombinant PD-1 coated ELISA plate.

2. Western Blot:   Recombinant human PD-1 can be detected with mAb clone 2H7.

3. IHC:   This antibody reacts with T-cells in thymus gland.  

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.