mAb anti-HCV NS-3, 20-8

Description: Mouse monoclonal antibody to human hepatitis virus C (HCV) non-structural protein NS-3

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Human hepatitis C virus (HCV) non-structural protein NS-3

Immunogen: A highly antigenic polypeptide consisting of essential sequences of at least 60 residues in length, which were selected from genes encoding the NS-3 region of a Chinese HCV strains

Fusion Myeloma: Sp2/0-Ag14

Specificity: React with synthetic HCV NS-3 protein (residues 1378-1458 on HCV polyprotein) 

Species Reactivity: Human hepatitis C virus, others not tested

Cross-Reactivity: No cross-reaction was found with HCV polyprotein core region, or other non-structural regions.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area: Virology

Background:  

Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission.  HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein).  The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2).  The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein.  HCV viral proteins are produced after processing the polyprotein.  Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.

Application: 

ELISA: Reacts with HCV NS-3.

Western Blot: mAb clone 20-8, when used at a concentration of 1μg/mL will allow visualization of 100ng/lane synthetic NS-3 protein. The mAb works on blots transferred from both reducing and non-reducing PAGE gel.

References: 

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.