mAb anti-human LIF, Clone 8B10

Description: Mouse monoclonal antibody to human Leukemia inhibitory factor

Purification: Protein G affinity purified

Product Type: Primary antibody- capture Primary antibody

Target Protein: LIF α subunit

Immunogen: Recombinant LIF α subunit

Fusion Myeloma: Sp2/0-Ag14

Specificity: Recombinant LIF Recombinant LIF α subunit

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distillated water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20^oC

Research Area: Cytokine and oncology

Background:  

LIF is a cytokine with important functions in diversified biological processes including cell differentiation, inflammatory response, neuronal development and cancer progression. LIF can induce the terminal differentiation of leukemia cells, thus it inhibits the further progression of leukemia. However, it has opposite effects on many other cancers, such as breast cancer and bladder cancer etc. Several recent studies have shown that LIF might play a significant role in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis development. Treatment of pancreatic cancer by blocking LIF is currently under investigation. Research also demonstrated that elevated LIF level in pancreatic tissue and circulation was correlated with the cancer progression, indicating that LIF might be useful as a biomarker.

Application: 

ELISA: This antibody is reactive to recombinant human LIF in indirect ELISA.

Western blot: This antibody detected recombinant LIF protein on Western Blot.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.