mAb anti-Human IL-8, I8-61

Description: Mouse monoclonal antibody to human interleukin 8 (IL-8)

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Human IL-8

Immunogen: Purified recombinant human IL-8

Fusion Myeloma: P3X63Ag8.653

Specificity: This antibody recognizes both recombinant and native human IL-8.

Species Reactivity: Human, others not tested.

Cross-reactivity: This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized in 0.01M PBS, pH 7.0.

Reconstitution: Double distilled water is recommended and to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20oC

Research Area: Cytokine, interleukin and chemotaxis

Background:

Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens.  IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens.  IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis.  IL-8 is also known to promote angiogenesis.

Applications:

ELISA: React with human IL-8.

Neutralizing: In chemotaxis assay, the antibody inhibited 97 % of the chemotactic activity of IL-8 on RB/293 cell using 5mg/ml of MAb and 10ng/ml of human IL-8 in assay.  This antibody inhibited the chemotactic effect of human IL-8, and had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating that the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.

Western Blot: The antibody when used at 0.02-0.1mg/ml concentration, will allow visualization of 100ng/lane of recombinant human IL-8.  Figure (see image): Western blot analysis of recombinant human IL-8 using clone I8-61.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.