mAb anti-Human IL-2, 6F11

Description: Mouse monoclonal antibody to human Interleikin-2 (IL-2)

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Human IL-2

Immunogen: Recombinant human IL-2 (15.5kDa)

Fusion Myeloma: Sp2/0-Ag14

Specificity: Reactive to recombinant human IL-2.

Species Reactivity: Human, cross-reactivity with other species has not been evaluated.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized in 0.01M PBS, pH 7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20oC

Research Area: Cytokine, adapted immunity

Background:

Interleukin 2 (IL-2) is the cytokine secreted by T-cells immediately after the T cell receptors are activated by the binding of foreign antigens.  The cytokine stimulates the growth, proliferation and differentiation of naïve T-cells into “effecter T-cells”, promotes the survival of antigen specific “memory T-cell”, and stimulates the maturation of “regulatory T-cells”.  The cytokine is crucial for the development of adapted immunity, immune memory and the modulation of auto-immunity.

Applications:

Fluorescence flow cytometry: Above (see image) is the histogram of Jurkat cells stained with 10ug/mL anti-human IL-2 mAb 6F11 and fluorescence labelled secondary antibody.  Black line histogram represents Jurkat cells stained with mouse IgG1 Kappa control antibody - anti-TB 38KD antigen clone B12F8 (Cat #: MO-I40021D) and fluorescence labelled secondary antibody.

ELISA: The antibody detects recombinant human IL-2 antigen in ELISA.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.