mAb anti Human IL-18 clone 10H4, Detector

Description: Mouse monoclonal antibody to human interleukin-18(IL-18)

Product Type: Primary antibody, detection antibody in matched antibody pair.

Purification: Protein G affinity purified

Target Protein: Human IL-18

Immunogen: Purified, human cell expressed recombinant human IL-18 

Fusion Myeloma: SP2/0

Specificity: Reactive with human cell expressed IL-18

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG2b Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to reconstitute the antibody to 1mg/mL.

Storage: Store at -20^oC

Research Area: Cytokine, inflammatory and auto-immune responses

Background:

IL-18, also known as interferon- inducing factor, is a pro-inflammatory cytokine produced by macrophage and other cells. Like interleukin-12, the expression of IL-18 can be stimulated by bacterial protein lipopolysaccharide (LPS) and can induce the production of interferon- by natural killer cells and certain T-cells. It has also been shown that IL-18 can inhibit the IL-4 dependent IgE and IgG1 production and increase IgG2a expression by B-cells. An interleukin-18-binding protein that is expressed by mononuclear cells, can bind IL-18 and inhibit its pro-inflammatory function. It has been reported that IL-18 is implicated in Hashimoto’s thyroiditis, age-related macular degeneration and Alzheimer’s disease.  

Applications: 

Sandwich ELISA: When Elisa plate is coated with IL-18 antibody clone 2A10 (Cat. No.: MO-C40094A), anti-human IL-18 antibody clone 10H4 can detect human IL-18 levels in Sandwich ELISA application.  

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.