mAb anti-Human PSA, CHYH2, Tracer (HRP Labeled), 0.2 mL

Description: Horseradish peroxidase (HRP) conjugated mouse monoclonal antibody to Human Prostate Specific Antigen (PSA)

Purification: Protein G affinity purified

Product Type: Tracer antibody in matched antibody pair, HRP conjugated

Target Protein: Human Prostate Specific antigen (PSA)

Immunogen: Human Prostate Specific antigen (PSA)

Fusion Myeloma: Sp2/0-Ag14

Specificity: This antibody is reactive to normal prostate, benign prostate hyperplasia and prostate malignant carcinoma. 

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG1 Kappa

Storage Buffer Formulation: Mixture of 50% glycerol and 50% PH7.2 0.01M PBS.  Final PH = 7.0 ± 0.1.

Storage: Store at -20^oC

Research Area: Oncology

Background:  

Prostate specific antigen (PSA) is a kallikrein related peptidase secreted by the epithelial cells of the prostate gland. Majority of PSA exists in plasma in bounded forms with α1 anti-chymotrypsin or other proteins. The protein is elevated in prostatic disorders such as prostate cancer and benign prostatic hyperplasia. PSA has been used as a biomarker for prostate cancer screening.

Application:

ELISA:  In combination with a monoclonal capture antibody clone CHYH1 (Cat. No.: MO-T40081A), this HRP conjugated antibody can be used as tracer antibody in sandwich ELISA applications for human PSA detection.

References:

1. Sadagopan Krishnan et al.  Attomolar Detection of a Cancer Biomarker Protein in Serum by Surface Plasmon Resonance Using Superparamagnetic Particle Labels* Angew. Chem. Int. Ed. Engl. 2011 February 1; 50(5): 1175–1178.

2. Vigneshwaran Mani et al.  Ultrasensitive Immunosensor for Cancer Biomarker Proteins using Gold Nanoparticle Film Electrodes and Multienzyme-Particle Amplification.  ACS Nano. Mar 24, 2009; 3(3): 585–594.

3. Mani V et al.  Highly efficient binding of paramagnetic beads bioconjugated with 100,000 or more antibodies to protein-coated surfaces.  Anal Chem. 2012 Dec 4;84(23):10485-91.

4. Bhaskara V. Chikkaveeraih et al.  Single-Wall Carbon Nanotube Forest Arrays For Immunoelectrochemical Measurement of 4 Protein Biomarkers for Prostate Cancer. Anal. Chem. Nov 1, 2009; 81(21): 9129-9134.

5. Bhaskara V. Chikkaveeraiah et al. Microfluidic Electrochemical Immunoarray for Ultrasensitive Detection of Two Cancer Biomarker Proteins in Serum.  Biosens Bioelectron. Jul 15, 2011; 26(11): 4477-4483.

6. Jeong-Mi Moon et al. A nanowire-based label-free immunosensor: Direct incorporation of a PSA antibody in electropolymerized polypyrrole. Biosensors and Bioelectronics. Volume 57, 15 July 2014, Pages 157–161.

7. Gary C. Jensen et al. Characterization of Multienzyme-Antibody-Carbon Nanotube Bioconjugates for Immunosensors.  J Nanosci Nanotechnol. Jan 2009; 9(1): 249–255.

8. Chikkaveeraiah BV, Mani V, Patel V, Gutkind JS, Rusling JF.Microfluidic electrochemical immunoarray for ultrasensitive detection of two cancer biomarker proteins in serum. Biosens Bioelectron 2011; 11(26):4477-83.