mAb anti-Human MCP-3, 113, Detector

Description: Mouse monoclonal antibody to human macrophage/monocyte chemotactic protein-3 (MCP-3)

Purification: Protein G affinity purified

Product Type: Primary antibody - Detection antibody

Target Protein: Human MCP-3

Immunogen: Recombinant human MCP-3 (M.W. 8.5kDa containing 76 amino acids residues)

Fusion Myeloma: Sp2/0-Ag14

Specificity: Reactive with natural and recombinant human MCP-3

Species Reactivity: Human, others not tested.

Cross-reactivity: Does not show any cross reaction with other human cytokines or growth factors tested such as IL-1β, IL-8, MCAF, SAA and EGF.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20oC

Research Area: Cytokine, chemotaxis

Background:

Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils.  MCP-3 showed 78% sequence homology with MCP-1 and 58% sequence homology with MCP-2.  The primary receptor for MCP-3 is D6.  MCP-3 also competes with MCP-1 to bind toCCR10, and competes with Rantes and MIP-1α to bind toCCR1. 

MCP-3 stimulates macrophage to secrete proteases.  A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasia.

Applications: 

ELISA: This antibody matches with capture antibody clone 542 (MO-C40088G) in sandwich ELISA.  

Western Blot: This antibody, when used at  concentration of 1-4μg/mL will allow visualization of 0.1μg/lane of human MCP-3.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.