mAb anti-Amyloid β Peptide 42, CA9 10C11, Detector

Description: Mouse monoclonal antibody to C-terminus of amyloid beta peptide 42 (Aβ42)

Purification: Protein G affinity purified

Product Type: Primary antibody, detection antibody in matched antibody pair.

Target Protein:  C-terminus f amyloid beta peptide 42

Immunogen: KLH conjugated to a short peptide (MVGGVVIA) with amino acid sequence corresponding to the C-terminal of Aβ42

Fusion Myeloma: Sp2/0-Ag14

Specificity: This antibody recognizes the C-terminal sequence (MVGGVVIA) of Aβ42 and full length Aβ42.

Cross-Reactivity: The antibody does not cross react with amyloid beta peptide 40 in dot blotting and ELISA. Cross-reactivity to amyloid beta peptide 43 is less than 1% in ELISA.

Species Reacitvity: Human and other primates; mouse, rat

Host / Isotype: Mouse, IgG2b Kappa

Formulation: Lyophilized from a solution in 0.01M PBS pH7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL. 

Storage: Store at -20^oC

Research Area: Aging and neurodegenerative diseases

Background:  

Amyloid beta peptide 42 (Aβ42) is best known for its role in the formation of senile plaques in the brain of patients with Alzheimer’s disease. Aβ42 and Aβ40 are the two major amyloid peptides that are produced after cleavage of amyloid precursor protein by secretases. Aβ42 (42 amino acids) is very fibrillogenic. The beta pleated structure of Aβ42 constituents the initial and key component of the insoluble amyloid fibril in senile plaque. It is widely accepted that Aβ42 contributes to the pathogenesis of Alzheimer’s disease. One proposition is that the deposition of amyloid fibril onto the brain tissue results in Alzheimer’s disease. Another is that the neurotoxicity of Aβ42 oligomer is the cause of the disease.

Application: 

1. ELISA: In combination with anti-amyloid peptide N-terminal capture antibody (mAb clone NT 5B8, Cat. No.: MO-M40094D), the antibody can detect Aβ42 in sandwich ELISA assay.

2. Western blot: The figure below is the result of using 2μg/mL anti-Aβ42 clone CA9 10C11 to detect Aβ42 on Western blot following Tris-Tricine separation gel electrophoresis. The antibody showed no cross-reactivity with Aβ40.  Lane 1 &2:  Aβ40, Lane 3&4: Aβ42.

3. Dot blot: The figure below is the result of using 1μg/mL anti-Aβ42 clone CA9 10C11 to detect 10ng/dot Aβ42 or Aβ40. The antibody showed no cross-reactivity with Aβ40.

References:

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