Description
Details
Description: Mouse monoclonal antibody to human interleukin-6 (IL-6)
Purification: Protein G affinity purified
Target Protein: Human IL-6
Immunogen: Purified recombinant human IL-6
Fusion Myeloma: F0
Specificity: The antibody is reactive with recombinant human IL-6. Reactivity with natural antigen has not been evaluated.
Species Reactivity: Human, others not tested.
Host / Isotype: Mouse, IgG2a Kappa
Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution: Double distilled water is recommended to adjust final concentration to 1 mg/mL.
Storage: Store at -20oC
Research Area: Cytokine, growth factor, inflammatory and autoimmune diseases
Background:
IL-6, also called B-cell stimulatory factor-2 (BSF-2), is an important cytokine for the differentiation of B-cells into immunoglobulin-secreting cells. IL-6 is mainly produced by T-cells, macrophages and endothelial cells in response to pathogenic molecular stimuli during infection, trauma and the growth phase of some types of cancer. IL-6 is up-regulated by the binding of Toll-like receptors to antigen pattern ligands. In acute inflammatory phase, IL-6 causes increased body temperature by initiating Prostaglandin E2 synthesis in hypothalamus.
Applications:
ELISA: The antibody reacts with recombinant IL-6 antigen.
Western blot: The antibody when used at 1:5000 dilution (0.2mg/mL) recognizes 5ng/lane recombinant human IL-6 (rhIL-6) on blot. Lane 1 and 2: rhIL-6 loaded at 5ng/lane. (See image)
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | mAb anti-Human IL-6, 8E10 |
---|---|
Application | EIA, WB |
Size | 0.1 mg |