pAb Rabbit anti-Mouse Kchip4

Description: Rabbit anti-Mouse potassium channel-interacting protein4 (Kchip4) polyclonal antibody

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Kchip4

Immunogen: A synthetic peptide (KTSSPAIQNSV) derived from the amino sequence acid residue 45-57 of mouse Kchip4, conjugated with KLH for immunization 

Specificity: This antibody is reactive to the 29kDa mouse Kchip4 in mouse brain tissue extract on Western blot.

Species Reacitvity: Mouse and other species with consensus  Kchip4 sequence (KTSSPAIQNSV)

Source: Rabbit

Formulation: Lyophilized in pH 7.4, 0.01M PBS

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20^oC

Research Area: Life science research, stem cell factor signalling, cancer research

Background:

KChIP4 is a member of the potassium channel interacting protein family.  It possesses calcium binding domains like other members of the KChIP family.  KChIP4 is cloned accidentally with the yeast two- hybrid method using Presenilin as bait.  The newly discovered protein does not affect the function of Presenilin, but instead, it will reconstitute the feature of A-type Kv channel after co-expression with Kv4.2, indicating that it is a potassium channel interacting protein. 

Application:

ELISA: Dilute the antibody approx. 1:300,000 for detection of Kchip4 peptide (KTSSPAIQNSV) coated on ELISA plate.

Western blot: Dilute approx. 1:4,000 for using in Western blotting to detect the 29kDa Kchip4 in mouse brain extract.

Fig.1 Western blot analysis of extracts from mouse brain using anti-kchip4 rabbit whole antiserum.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.