High Sensitivity Human CRP ELISA Kit

RACTIVITY	Human
SENSITIVITY	0.005 mg/L
ASSAY RANGE	0.005 mg/L- 10 mg/L
REAGENTS PROVIDED	CRP MICROTITER PLATE  CRP CONJUGATE CRP STANDARD SET TMB SUBSTRATE STOP SOLUTION CRP SAMPLE DILUENT

INTENDED USE

This High Sensitivity Human CRP ELISA kit is to be used for the in vitro quantitative determination of human c reactive protein (CRP) concentrations in serum, plasma and cell culture supernatant.  This kit is intended FOR LABORATORY RESEARCH USE only and not for use in diagnostic or therapeutic procedures.

INTRODUCTION

Human CRP is a kind of nonimmunoglobulin serum substance, a heat labile b-globulin.  It is classified in a superfamily of proteins termed pentaxins or pentraxins: cyclic, non-glycosylated structures composed of five apparently identical globular non-covalently linked subunits aggregated symmetrically.  Each subunit is 23.05 kD (206 amino acids), with a total molecular weight of 117.5 kDa, and consists of 14 anti-parallel b-strands arranged in two b-sheets.

CRP is an acute phase protein, originally identified and named for its ability to precipitate the C-polysaccharide of pneumococcus in the presence of calcium.  It is the prototypic acute phase reactant whose presence in plasma or serum serves as a useful laboratory indicator of systemic inflammatory disease.  Normally, CRP in human biological fluids is present in trace amounts (0.07-8.00 mg/L, median 0.6 mg/L). Stimulated by certain cytokines (IL-1a, IL-1b, TNF-a and b, and indirectly by IL-6), its synthesis by hepatocytes enhanced dramatically.  During the acute phase response, CRP concentration can increase up to 1000-fold within a few hours.

Among acute phase proteins, CRP is a fast-reacting, sensitive and the most easily measured one.  It has a rapid response time, short half-life and large incremental change and its catabolism is not affected by the type of inflammation.  Following acute tissue damage or during the course of infectious and non-infectious conditions, hepatic synthesis of CRP dramatically increases.  Typically, mild elevations of CRP are seen in a variety of inflammatory conditions. Serum amyloid A (SAA) is another major acute phase protein whose response is highly correlated with that of CRP.  Both CRP and SAA respond sensitively to several stimuli, but they differ in certain responses.

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