pAb Rabbit anti-Bcl-2

Description: Rabbit anti-B cell lymphoma factor 2 (Bcl-2) polyclonal antibody

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Bcl-2

Immunogen: A synthetic peptide (AGRTGYDNREIVMKY) derived from human Bcl-2, conjugated with KLH for immunization

Specificity: This antibody recognizes the synthetic Bcl-2 peptide. 

Species Reacitvity: Human and other species with consensus Bcl-2 sequence (AGRTGYDNREIVMKY)

Source: Rabbit

Formulation: Lyophilized in pH 7.4, 0.01M PBS

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area: Life science research, Oncology, apoptosis signalling pathway

Background:

Bcl2 is a 25kDa inner mitochondrial membrane protein with anti-apoptotic function.  It is believed that Bcl-2 suppresses apoptosis by inhibiting mitochondrial cytochrome C release, blocking caspase cascade and regulating mitochondrial calcium homeostasis.  Bcl-2 is identified from the study of B-cell lymphoma.  About 90% of follicular lymphoma is caused by Bcl-2 over-expression resulted from the translocation of Bcl-2 gene from chromosome 18 to the immunoglobulin heavy chain enhancer on chromosome 14.  Bcl-2 is also involved with other malignancies such as melanoma, breast cancer, prostate cancer and lung cancer.  

Application:

ELISA:  React with the synthetic Bcl-2 peptide coated on ELISA plate.   

Other applications have not been evaluated.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.