mAb anti-Human AFP, AFP-Y2, Detector

Description: Mouse monoclonal antibody to human alpha fetoprotein (AFP)

Purification: Protein G affinity purified

Product Type: Primary antibody - detection antibody in matched antibody pair

Target Protein: Human AFP

Immunogen: Human AFP, which was isolated from amniotic fluid and purified by affinity chromatography.

Fusion Myeloma: Sp2/0-Ag14

Specificity: React with AFP.  No detectable cross-reaction with human normal serum proteins. 

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH7.0

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area: Oncology

Background:  

Alpha fetoprotein (AFP) is a 70kD glycoprotein which is produced primarily by liver cells in foetuses. It exists in significant amount in foetal plasma and decreases rapidly after birth. Moderate amount of AFP exists in serum of pregnant women. Monitoring mother’s serum AFP level in second trimester pregnancy can help assessing birth defects such as Down syndrome, neural tube defect and anencephaly. Elevated AFP level in men, children and non-pregnant women is associated with neoplastic conditions in lever, testicle or ovary.

Application: 

ELISA:  This antibody can be used as a detection antibody in sandwich immunoassay for human AFP detection in combination with capture antibody mAb AFP-Y1 (Cat. No.: MO-T40007B). 

Western blot: The antibody identifies 68kD AFP on western blot.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.